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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in . The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
A new chiral derivatizing reagent, dehydroabietylisothiocyante (DHAIC), was synthesized and used for the enantiomeric separation of chiral compounds in capillary electrophoresis (CE). The synthetic route to obtain DHAIC is described. The separation conditions for the chiral separation of several chiral compounds, such as protein amino acids and chiral drug DOPA were optimized. Best results for the chiral separation of DHAIC derivatized amino acids and DOPA were obtained in a running buffer consisted of 50mM borate (pH 9.5), 5mM sodium dodecyl sulphate (SDS) and 20% acetonitrile for amino acids and 60mM Na"2HPO"4 (pH 8.0), 17mM SDS and 25% acetonitrile for DOPA. Under the conditions studied, chiral separation of five amino acids including Ser, Val, Ala, Thr, Cys and a chiral drug DOPA as their diastereomeric DHAIC derivatives has been achieved by micellar electrokinetic chromatography (MEKC). .
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This digital document is a journal article from Analytica Chimica Acta, published by Elsevier in 2004. The article is delivered in HTML format and is available in your Amazon.com Media Library immediately after purchase. You can view it with any web browser.

Description:
A new thiol-reactive derivatizing reagent, 3-iodoacetylaminobenzanthrone (IAB) has been developed for thiol analysis in liquid chromatography. In aqueous methanol containing 15mM pH 8.3 H"3BO"3-KCl-Na"2CO"3 buffer, IAB reacted with thiols at 35^oC for 15min. The derivatives of IAB with glutathione (GSH), cysteine (Cys), homocysteine (Hcy) and N-acetylcysteine (Nac) were well separated on a C"1"8 column with the mobile phase of methanol-water (50:50, v/v) containing 15mM pH 2.7 H"3cit-Na"2HPO"4 buffer. At @l"e"x/@l"e"m=420/540nm, the detection limits were 20, 20, 55 and 40fmol (1, 1, 2.3 and 2nM), respectively, with a signal-to-noise ratio of 3. Owing to the preferential selectivity of iodoacetamidyl moiety to @?SH group, amino acids, aliphatic amines, phenol and alcohols had no obvious interference with the determination. The proposed method has been applied to the determination of thiols in human blood with recoveries of 98.5-105.3%. .
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