Books about Interfering from Amazon.com

RNA interference has become a key method in the suppression of gene expression and the development of therapeutic agents, yet there is still the problem of delivery, stability, and the danger of off-target effects such as the silencing of unwanted genes and activation of innate immunity. In siRNA and miRNA Gene Silencing: From Bench to Bedside, expert researchers explore the most recent advances in siRNA design, expression, delivery, in vivo imaging, and methods to minimize siRNA’s unwanted effects and promote successful use in patients. As part of the highly successful Methods in Molecular Biology™ series, the chapters focus on their respective subjects with easy-to-use, up-to-date information, including several step-by-step laboratory protocols on topics such as new delivery formulations and strategies with promising applications in vitro and in vivo, validated therapeutic target genes, and components of miRNA function, biogenesis, and interference with virus infection.

Comprehensive and cutting-edge, siRNA and miRNA Gene Silencing: From Bench to Bedside offers an excellent collection of chapters to aid all those with an interest in RNAi, gene regulation, and new therapies.

.
Price: $93.54 [Notify me when price goes down.]

Providing a comprehensive overview of a fast-emerging field, this volume dedicated to the biological role of microRNAs (miRNAs) discusses methods for identifying and characterizing their functions and looks at potential applications of microRNA research in medicine and pharmacology. miRNAs are small, noncoding RNA molecules that appear to regulate gene expression. Found in plant and animal species and possibly traceable back to ancient forms of life, miRNAs are now estimated to regulate a third or more of genes in the human genome. The Editors have included material from some of the very best, most active and upcoming researchers in the field, to provide the reader with a rounded overview of the biology, function and expression of miRNAs. The chapters are grouped according to the following themes: Ø Basic miRNA Biology, Ø General Characteristics of miRNAs, Ø Species Interest and Function, Ø miRNA Detection Technologies and finally, Ø Bionformatics Approaches to Identification of miRNAs and miRNA targets. This book is a valuable tool for students, university instructors, undergraduate and graduate biology students, and experienced researchers..
Price: $35.95 [Notify me when price goes down.]

In recent years Molecular Biology has experienced an unprecedented revolution by the discovery of functional small RNAs. The number of cellular processes in which non-coding RNAs are involved is growing rapidly and include gene regulation on the transcriptional, post-transcriptional and translational level. To complicate matters, these processes seem to be strongly interconnected on the one hand, and diverse among different organisms on the other. This volume describes strategies for the discovery and validation of small RNAs and provides a snapshot of our current understanding of the different mechanisms triggered by small RNAs.

.
Price: $29.99 [Notify me when price goes down.]

A collection of readily reproducible methods for the design, preparation, and use of RNAs for silencing gene expression in cells and organisms The techniques range widely and include methods addressing the biochemical aspects of the silencing machinery, RNA silencing in non-mammalian organisms, and the in vivo delivery of siRNAs and silencing vectors. There are also techniques for designing, preparing, and using RNAs to silence gene expression, for fine-tuning regulation by targeting specific isoforms of a given gene, and for the study and use of microRNAs. The protocols follow the successful Methods in Molecular Biologyâ„¢ series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls..
Price: $50.53 [Notify me when price goes down.]

RNA Interference (RNAi) technology has rapidly become one of the key methods used in functional genomics. RNAi is used to block the expression of genes and create phenotypes that can potentially yield clues about the function of these genes. In the postgenomic era, the elucidation of the physiological function of genes has become the rate-limiting step in the quest to develop 'gene-based drugs' and RNAi could potentially play a pivotal role in the validation of such novel drugs. In this cutting-edge overview, the basic concepts of RNAi biology are discussed, as well as the current and potential applications. Leading experts from both academia and industry have contributed to this invaluable reference for graduate students, post-docs and researchers from academia wanting to initiate RNAi research in their own labs, as well as for those working in research and development in biotech and pharmaceutical companies who need to understand this emerging technology. Forwarded by Andrew Fire, one of the winners of the 2006 Nobel Prize for the discovery of RNA Interference..
Price: $150.00 [Notify me when price goes down.]

Nearly 97% of the human genome is the non-coding DNA, which varies from one species to another, and changes in these sequences are frequently noticed to manifest clinical and circumstantial malfunction. Numerous non-protein-coding genes are recently found to encode microRNAs, which are responsible for RNA-mediated gene silencing through RNA interference (RNAi)-like pathways. MicroRNAs (miRNAs), small single-stranded 17–25 nucleotide RNAs capable of interfering with intracellular messenger RNAs (mRNAs) that contain either complete or partial complementarity, are useful for the design of new therapies against cancer polymorphism and viral mutation. Currently over 1000 native miRNA species found in vertebrates and many more new miRNA homologs continue to be identified; however, most of their functions remain to be determined. In this book, many new perspectives of the miRNA research are reviewed and discussed, including their roles in stem cell maintenance, embryonic development, tissue differentiation, adult physiology, disease pathology, cancer research, viral infection, genetic engineering in plants, and utility in cosmetic applications. These new findings may not only provide significant insight into the various mechanisms of miRNAs but also offer a great opportunity in developing new therapeutic interventions.

.
Price: $161.44 [Notify me when price goes down.]

One of the major recent discoveries in molecular and cellular biology is that small double-stranded RNA molecules selectively turn off gene expression in all types of cell, a phenomenon known as RNA silencing. This discovery led to the development of RNA interference (known as RNAi) as a powerful research tool in the functional study of individual genes and their products, and in functional genomics. In RNAi, specific small double-stranded RNA molecules (small interfering RNAs or siRNAs) are introduced into cells to selectively silence certain genes."RNAi "covers the basic concepts and mechanisms of RNAi, transfection of cells with siRNAs, the design and validation of RNAi reagents, RNAi techniques in different organisms, large-scale RNAi screening, applications of RNAi in drug discovery, and potential uses of RNAi as a therapeutic agent. A key feature of "RNAi" is the highlighting of the pitfalls that can occur and how to minimize them. The book also contains a complete list of abbreviations, as there are many confusing terms associated with RNAi..
Price: $64.05 [Notify me when price goes down.]

Electronic Noise and Interfering Signals is a comprehensive reference book on noise and interference in electronic circuits, with particular focus on low-noise design. The first part of the book deals with mechanisms, modelling, and computation of intrinsic noise which is generated in every electronic device. The second part analyzes the coupling mechanisms which can lead to a contamination of circuits by parasitic signals and provides appropriate solutions to this problem. The last part contains more than 100 practical, elaborate case studies. The book requires no advanced mathematical training as it introduces the fundamental methods. Moreover, it provides insight into computational noise analysis with SPICE and NOF, a software developed by the author. The book addresses designers of electronic circuits as well as researchers from electrical engineering, physics, and material science. It should also be of interest for undergraduate and graduate students.

.
Price: $126.44 [Notify me when price goes down.]

In this completely updated and expanded edition of a classic bench manual, hands-on experts take advantage of the latest advances in ribozyme, DNAzyme, and RNA interference technologies to describe in detail the exciting and successful methods now available for gene inactivation in vitro and in vivo. Their optimized techniques employ hairpain ribozymes, DNAzymes, hammerhead ribozymes and derivatives, group I intron ribozymes, Rnase P ribozymes, and siRNAs, as well as general methods for RNA structure analysis, delivery of oligonucleotides, and gene therapy. Also provided are novel methods for identifying accessible cellular mRNA sites; group I intron and RNAse P ribozymes protocols for effective design, selection, and therapeutic applications; and the latest RNAi methods for sequencing-specific gene silencing in a wide variety of organisms. Comprehensive and up-to-date, Ribozymes and siRNA Protocols synthesizes for experienced and novice investigators alike the exciting advances in understanding nucleic acid enzymes and demonstrates how they may be used to analyze gene function and target validation, and to productively develop new therapeutics for human diseases..
Price: $82.04 [Notify me when price goes down.]

MicroRNAs (miRNA) are tiny bits of genetic material that were unknown nearly 10 years ago but now represent an exciting field of study in biology. Upon their discovery, researchers revealed for the first time a new mechanism by which microRNA can stop the function of messenger RNA (mRNA) by literally cutting it in half, interfering with the normal function of specific messenger RNAs in gene expression.

This "expression" of genes that code for essential proteins is essentially what controls whether a cell turns into a liver, lung, or brain cell, for example. Understanding what activates this process - or stops it - is a key to understanding the biological process and builds a foundation for advances in medicine and other fields. This volume in Methods in Enzymology presents valuable methods for studying MicroRNA, with three sections covering identification of MicroRNAs and their targets; Micro RNA expression, maturation and functional analysis; and MicroRNAs and disease..
Price: $149.95 [Notify me when price goes down.]