Books about Monoclonal from Amazon.com

It began with a promising cancer drug, the brainchild of a gifted researcher, and grew into an insider trading scandal that ensnared one of America's most successful women. The story of ImClone Systems and its "miracle" cancer drug, Erbitux, is the quintessential business saga of the late 1990s. It's the story of big money and cutting-edgescience, celebrity, greed, and slipshod business practices; the story of biotech hype and hope and every kind of excess.

At the center of it all stands a single, enigmatic figure named Sam Waksal. A brilliant, mercurial, and desperate-to-be-liked entrepreneur, Waksal was addicted to the trappings of wealth and fame that accrued to a darling of the stock market and the overheated atmosphere of biotech IPOs. At the height of his stardom, Waksal hobnobbed with Martha Stewart in New York and Carl Icahn in the Hamptons, hosted parties at his fabulous art-filled loft, and was a fixture in the gossip columns. He promised that Erbitux would "change oncology," and would soon be making $1 billion a year.

But as Waksal partied late into the night, desperate cancer patients languished, waiting for his drug to come to market. When the FDA withheld approval of Erbitux, the charming scientist who had always stayed just one step ahead of bankruptcy panicked and desperately tried to cash in his stock before the bad news hit Wall Street.

Waksal is now in jail, the first of the Enron-era white-collar criminals to be sentenced. Yet his cancer drug has proved more durable than his evanescent profits. Erbitux remains promising, the leading example of a new way to fight cancer, and patients and investors hope it will be available soon.

.
Price: $2.77 [Notify me when price goes down.]

With revenues from the top five therapeutic antibodies accounting for a majority of the recent pharmaceutical sales, the research and development in the field has exploded over the past several years and is expected to grow with new emerging monoclonal antibodies like Numax, Lucentis, Actemra, and others. In Therapeutic Antibodies: Methods and Protocols, leading experts from academic laboratories and biotechnology companies present an extensive set of protocols for the discovery and development of therapeutic antibodies, featuring sections devoted to recombinant antigens, antibody libraries, antibody discovery, antibody engineering, and antibody preclinical development. Written in the highly successful Methods in Molecular Biologyâ„¢ series format, the chapters contain brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Therapeutic Antibodies: Methods and Protocols serves as a key resource for researchers and antibody engineers investigating and participating in this rapidly growing therapeutic market segment.

.
Price: $93.73 [Notify me when price goes down.]

Monoclonal Antibodies: Methods and Protocols examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting with opening chapters focusing on the gold standard method for generating mouse monoclonal antibodies through hybridoma technology, future methods for engineering recombinant and humanized antibodies, methods for engineering soluble Fc fusion protein, and the use of antibodies and flow cytometry in the quantification of cell signaling proteins.

Specific chapters describe how antibodies are used for the diagnosis and classification of hematologic diseases. Subsequent chapters examine the advantages and most recent advances of using bead-based immunoassays, including the ability of bead-based technology to multiplex and analyze several analytes simultaneously, and the use of beads in detecting fusion proteins resulting from chromosomal translocations. Concluding chapters provide additional examples of methodologies that employ monoclonal antibodies.

Monoclonal Antibodies: Methods and Protocols provides descriptions of methods that cover a wide spectrum of applications in the field of monoclonal antibodies. This field will continue to expand and provide new and innovative techniques, not only in the laboratory, but also as a basis that complements targeted therapy.

.
Price: $57.95 [Notify me when price goes down.]

Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.
The Third Edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an integrated treatment of both the production and application of monoclonal antibodies. As in previous editions, detailed and critical accounts of the theory, production, purification, fragmentation, storage and radiolabelling of monoclonal antibodies are given, along with descriptions of their use in antigen characterization, affinity chromatography and immunofluorescence. The present volume has been comprehensively updated to cover recent rapid advances, particularly with respect to the applications of molecular biology, the use of antibodies in closing and heterologous expression of genes, immunohistology and phage display libraries. Since the previous edition, there has been a growing trend towards the replacement of procedures using radioactive isotopes, and the current edition incorporates these newer technologies.
The text is oriented towards problems solving, and makes it easy to adapt each procedure to individual needs. Extensive cross-referencing, a glossary and a comprehensive index make this book an essential reference.
This book will be vital both for laboratories already producing or using monoclonal antibodies, and for workers in many disciplines who are contemplating their use.

*
* Provides an integrated treatment of both the production and application of monoclonals in cell biology, biochemistry, and immunology.
* Gives detailed and critical accounts of the theory, production, purification, storage, and relabelling of monoclonals, and their use in antigen characterization, affinity chromorography, and immunofluroscence.
* Comprehensively updated to cover the rapid advances that have occurred since the publication of the Second Edition..
Price: $74.00 [Notify me when price goes down.]

The last three decades have provided opportunities to explore the potential of treating malignant diseases with antibodies or other targeting molecules labelled with nuclides. The expanding array of new targeting molecules (recombinant antibodies or peptides) may increase the therapeutic efficacy. The title of this book "Targeted Radionuclide Tumor Therapy – Biological aspects" was selected to reinforce the concept that a major focus was devoted to understanding the biological effects of targeting and radiation. Furthermore, our rapidly expanding knowledge of low dose-rate effects, different types of cell death, autosensitization and the increasingly likely existence of cancer stem cells suggests to us that even more efficient approaches in targeting might be possible in the future.

The development of targeted therapy is a true multidisciplinary enterprise involving physician scientists from the fields of nuclear medicine, radiation therapy, diagnostic radiology, surgery, gynaecology, pathology and medical oncology/haematology. It also involves many preclinical scientists working with experimental animal models, immunochemistry, recombinant antibody technologies, radiochemistry, radiation physics and basic cell biology.

It is the ambition of the editors to enable deeper insights in the process of improving targeted therapy. We believe that the time now has come when targeted therapy can soon be added to standard oncology treatment regimens.

.
Price: $198.48 [Notify me when price goes down.]

Exciting work in the past decade has revealed commonalities and differences among individual cell fusion events. In Cell Fusion: Overviews and Methods, a team of leading experts provide a collection of overviews that outline our current understanding of cell fusion and methods that present classic and state-of-the-art experimental approaches in a variety of systems.  Divided into two convenient parts, the volume begins with nine overviews which describe different cell fusion events in models from yeast to mammals, and it continues with thirteen chapters illustrating commonly used methods to assay cell fusion in particular systems. As a part of the highly successful Methods in Molecular Biology™ series, these methods chapters compile step-by-step, readily reproducible protocols with lists of the necessary materials and reagents, along with tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and user-friendly, Cell Fusion: Overviews and Methods serves as a comprehensive resource for anyone, expert or novice, interested in the fascinating biological process of cell fusion.

.
Price: $74.39 [Notify me when price goes down.]

FROM THE PREFACE: Several papers in this volume deal with advances in hybridoma technology and complement the chapter by Galfri and Milstein that appeared in Volume 73 of this series. Methods to enhance the efficiency of producing desired fusion products, to simplify the basic procedures involved, and to assess the specificity and other properties of monoclonal antibodies are discussed. Representative examples illustrate how a battery of monoclonal antibodies with different specificities can be used to study various immunochemical and biochemical problems. Other chapters cover additional procedures that can be used to label antigens and antibodies, separate antigen-antibody complexes, and process data. Some papers classified under one heading or contained in another volume of the series include methods that can be applied in other specific areas. For example, cytotoxicity tests described in Volume 93 may be used to screen supernatant fluids in hybridoma experiments if cytotoxic activity is a desirable property of the monoclonal antibody..
Price: $264.73 [Notify me when price goes down.]

This book is among the first to combine technical and clinical aspects of the subject. It aims to provide a unique combination of the production (by both cellular and molecular biology techniques), structure and functional characteristics of monoclonal antibodies, together with detailed discussions of the various analytic, diagnostic and therapeutic applications of these antibodies. Many areas of clinical medicine benefit from this type of study including histopathology, oncology, transplantation, infectious diseases, rheumatology, haematology, and dermatology..
Price: $69.56 [Notify me when price goes down.]

This successful laboratory handbook focuses on procedures for hybridoma generation and gives detailed test protocols for the application of monoclonal antibodies. Immunization, cell preparation, hybridization, cell culture and cloning of hybridomas, prevention of back-mutation, as well as purification, labeling, and characterization of the antibodies obtained, are only a few keywords regarding the contents. Practical hints and detailed product information together with detailed protocols for trouble-shooting make this book an exceptionally useful "cookbook" as well as a compendium of immunological methods over and above monoclonal antibody technology. The authors have emphasized protocols which tend to minimize the number of animals used and favor methods which avoid radioactivity..
Price: $57.00 [Notify me when price goes down.]

Emerging technologies in target identification, drug discovery, molecular markers, and imaging are rapidly changing the face of cancer. Targeted Cancer Therapy provides a foundation of knowledge in targeted cancer therapeutics. The treatment of cancer is increasingly being individualized, based on an understanding of underlying biologic mechanisms. Poised to change the landscape in oncology, Targeted Cancer Therapy provides a state-of-the-art overview. Targeted Cancer Therapy is valuable to practicing and academic physicians, fellows, residents and students, as well as basic scientists, interested in the cancer field.

.
Price: $115.70 [Notify me when price goes down.]